cid acid (C22:0, m/z = 412), etracosanoic octadecenoic acid (C18:1, m/z = 354), octadecanoic acid acid (C20:0, m/z = 384), docosanoic (C20:0, m/z = 384), docosanoic acid acid (C24:0, = 412), etracosanoic acid (C24:0, m/z = 440),468), octacosanoic acid (C28:0, m/z = 496), triacontanoic acid (C30:0, (C22:0, m/z m/z = 440), hexacosanoic acid (C26:0, m/z = hexacosanoic acid (C26:0, m/z = 468), octacosanoic acid (C28:0, m/z = 524). m/z = 496), triacontanoic acid (C30:0, m/z = 524).three.five. GC-MS Analyses of Compounds Extracted from AdultsTable 5. Fatty acid content from the cuticular and CXCR Antagonist site internal lipids extracted from Sarcophaga argyrostoma adults ( /g of insect The untreated adults contained, respectively, three.0- and 3.4-times larger total masses body SD).FFA Hexanoic acid C6:0 Heptanoic acid C7:0 Octanoic acid C8:0 Nonanoic acid C9:0 Decanoic acid C10:0 Undecanoic acid C11:0 Dodecenoic acid C12:1 Dodecanoic acid C12:0 Tridecanoic acid C13:0 Tetradecenoic acid C14:1 Tetradecanoic acid C14:of FFAs within the cuticular and internal extracts compared to the untreated pupae. In contrast towards the pupae,Cuticular the exposure of adult flies to C. coronatus resulted in elevations in the total Internal FFA masses in both the cuticular (1.7-fold) and internal fractions (1.1-fold). Exposure to Exposure for the a comparable ETB Antagonist supplier Controlcontrol adults typically demonstratedControl cuticular FFA profile to the conC. coronatus C. coronatus trol pupae; even so, the adults lacked C19:0, but integrated eight FFAs, which were absent A 0.06 0.00 A 0.09 0.01 A 0.04 0.02 0.00 A from the pupae (C11:0, C12:1, C15;1, C18:three, C19:1, 0.00 C20:3, and C24:1). Of your FFAs C29:4, 0.02 were B 0.04 the A,B 0.02 and untreated adults, C14:0 was 25-fold 0.01 0.00 B that 0.00 abundant to each 0.01 untreated pupae 0.00 A extra plentiful inside the adults,0.01 A,B was 11-fold, C17:1 was eight-fold, C18:2 was six-fold, 0.04 0.00 B 0.08 C16:1 0.03 0.00 A 0.02 0.00 A,B and C20:5 B was 20-fold much more plentiful when compared with theA pupae. In contrast, the following 0.09 0.01 0.16 0.01 A,B 0.08 0.01 0.04 0.00 A,B significant differences have been found between the internal FFAs of your manage adults along with the 0.02 0.00 B 0.03 0.00 A,B 0.02 0.00 A trace quantity A,B handle pupae: thirteen FFAs have been present within the adults that had been absent within the pupae 0.05 0.01 A ND A,B 0.05 0.00 C30:0, and (C14:1, C17:1, C17:0, 0.16 0.01 A,B C20:4, C20:0, C22:0, C24:0, C26:0, C28:0, B C18:3, C20:5, C32:0); on the 0.01 0.00 A,B abundant internal FFAs, C6:0 was 18-fold additional abundant 0.00 A,B ND B ND A 0.01 inside the pupae, C7:0 was seven-fold, C8:0 was 11-fold, and C9:0 was eight-fold far more abundant than in the 0.09 0.00 A 0.34 0.01 A 0.04 0.01 A 0.07 0.00 A adults, although C16:1 was 23-fold much more abundant inside the adults, C18:two was eight-fold, plus a,B ND A 0.18 abundant than inside the pupae (Tables three and five).0.00 A,B ND B 0.03 C18:1 was four-fold extra 0.02 A A A 0.25 0.01 adults, the exposure to C. coronatus resulted in the disappearance of C12:1 and 0.42 0.01 0.07 0.01 0.ten 0.01 A Inside the C32:0 from 0.82 0.00 A the cuticle, as well as the appearance of C13:0 and C24:1. Exposure was also associ2.78 0.14 A,B 0.33 0.01 A,B 0.97 0.00 B ated with a rise in the concentration of 20 FFAs (C6:0, C8:0, C9:0, C11:0, C12;0, C14:1, C14:0, C15:1, C15:0, C16:1, C16:0, C17:0, C18:2, C18:1, C18:0, C20:five, C20:4, C20:0, C22:0,Insects 2021, 12,12 ofTable five. Cont. Cuticular FFA Pentadecenoic acid C15:1 Pentadecanoic acid C15:0 Hexadecenoic acid C16:1 Hexadecanoic acid C16:0 Heptadec
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