E of exosome, which was proven to be incredibly convenient and trusted. We believe that this approach is extremely efficient and economical and has good potential to become additional utilized for the selective separation of exosome. Funding: This function was supported by a National Analysis Foundation (NRF) grant funded by the Korean government, Ministry of Education and Science Technology (NRF-2017M2A2A6A01071157, NRF-2018R1C1B6008799).JOURNAL OF EXTRACELLULAR VESICLESPF10.11=OWP3.Aqueous two-phase method to isolate extracellular vesicles for prostate cancer diagnosis Hyunwoo Shina, Jiyoon Kima, Mee Young Kimb, Yong Hyun Parkb, Yong Goo Kimc, Ji Youl Leeb and Jaesung Parkd POSTECH, Pohang, Republic of Korea; bDepartment of Urology, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul, Republic of Korea, Seoul, Republic of Korea; cDepartment of Laboratory Medicine, Mary’s Hospital, The Catholic University of Korea, Seoul, Republic of Korea, Seoul, Republic of Korea; dDepartment of Mechanical Engineering, POSTECH, Pohang, Republic of Korea, Pohang, Republic of KoreaaIntroduction: Analyzing extracellular vesicles (EVs) is definitely an desirable means in prostate cancer diagnosis. Nonetheless, current solutions of EVs isolation have low efficiency, purity and lengthy approach time, which induce low CD1a Proteins Recombinant Proteins diagnostic potential. To method the challenges, we adapt a two-phase method to diagnose prostate cancer by isolating EVs from patients’ urine. Applying the twophase system, prostate hyperplasia (BPH) sufferers and prostate cancer (PCA) patients had been diagnosed, and also the diagnostic capacity was compared with conventional diagnostic procedures. Strategies: Forty-two prostate cancer (PCA) individuals and 20 benign prostate hyperplasia (BPH) patients’ urine, plasma, saliva was collected and applied for identifying EVs isolation capacity of aqueous two-phase technique(ATPS) and for comparing diagnostic capability of ATPS with standard diagnosis. Final results: With an optimized ATPS, EVs have been isolated with an efficiency of about 90 . Additionally, the EV-isolation time was within about 30 min, plus the purity of EVs in ATPS was roughly two occasions improved than achieved using a standard strategies, ultracentrifugation and polymeric precipitation. Right after the ATPS isolated EVs from patients’ body fluid, PCR and ELISA had been utilized to detect EVs derived from prostate cancer cells. The expression levels of RNA and protein markers of prostate cancer were compared, and also the partnership involving expression levels and clinical data was analysed. The results demonstrated that diagnostic capacity according to ATPS was far better than other conventional procedures (serum PSA and sediments). Furthermore, sensitivity enhanced by no less than ten , and specificity was improved by at the least 20 in comparison with standard approaches. Summary/conclusion: Good quality and quantity of EVs might be obtained from patients’ physique fluid utilizing ATPS. Utilizing the abundant sources, which include cancer-related proteins and genes, we are able to perform a diagnosis with higher specificity and sensitivity. Therefore, ATPS delivers a powerful tool for far more distinct and sensitive diagnosis.CD45 Proteins Gene ID ISEV2019 ABSTRACT BOOKPF11: EV-Based Therapeutics II Chairs: Yasnouri Fujita; Xue Zou Place: Level 3, Hall A 15:306:PF11.Therapeutic effect of plant sap-derived nanovesicles on cancer cells Kim Kimina, Yeon Ju Hunb, Yoo Hye Jua and Ruri LeeaaUniversity of brain education, Cheon-an, Republic of Korea; bUniversity of British Columbia, cheonan, Republic of KoreaIntroduction: Mo.