Ome hydrophobic dyes that aid in purification when on the 5′ end of an oligo (Cy3, Cy5, Dabcyl, TAMRA) tend to challenge 5′-DMT specificity during purification when placed internally or at the 3′ end. Additionally, any DMT-ON failure sequences derived from a stepwise synthesis cycle or 8
GLEn-PAkTM OPTIOnS
The advantages of using the 96-well format include: Lower cost processing (less labor and unattended operation if using a robot). Less processing time per oligo when using a whole plate. Less solvent usage for every purification step. Less final elution volume to dry in preparation for analysis or use. Glen-Pak DNA 50mg Purification Cartridge (60-5000-96) – Up to 200 nmole Capacity Introduced primarily for those using automated liquid handlers. It has a capacity of up to a 200 nmole scale and the same 96 well, standard configuration as the regular sized Glen-Pak DNA cartridge. The main benefit for those using liquid handlers is the shorter bed height, which allows for lower volume, single reagent additions at most of the purification steps. This cartridge is only offered in the vacuum manifold configuration due to its intended use with higher throughput applications. Some of the benefits of the 50mg cartridge include: Lower bed volume leaves more room for reagents in the column. Lower volume additions for all but the DMT removal step. Lower final elution volume of 0.5mL, which reduces sample drying time. More amenable to robotic applications due to single reagent addition capability. Glen-Pak DNA Purification Cartridge (605100-xx, 60-5200-xx) – Up to 1 ole Capacity Ideal for DMT-ON based purification of oligonucleotides up to a 1.0 ole scale and comes in both hand held syringe and manifold compatible configurations. The vacuum style cartridges utilize a standardized adapter rack to allow use of 96 well manifolds and due to their luer tip, are also compatible with multi port chamber style manifolds. The syringe style gives a single use or lower throughput option with no change in protocol. Methods have been developed for 2′-OMe, 2′-F, and phosphorothioate oligonucleotides as well as for simple desalting of both DNA and RNA. Additional methods for 5′-Thiol, 6-FAM, CyDye and phosphorylated oligos can be found in the Glen-Pak User Guide.
instrument issues may co-purify with the full-length product. Finally, as with any chromatographic technique, challenging sequence content of an oligonucleotide such as G-quartets or designed hairpins can dramatically impact binding of the 5′-DMT to the Glen-Pak resin.635702-64-6 IUPAC Name As such, the Glen-Pak performance is directly correlated to the quality of the synthesis itself as well as the direct composition of the oligonucleotide.838818-26-1 Formula VERsATIlITy, TIPs AND TRIcks In response to customer requests, we have added protocols to the Glen-Pak User Guide for many different oligo types.PMID:28613699 Some tips have also emerged as helpful in the routine use of Glen-Paks, as noted below. For example, the protocol for purification of phosphorothioates as well as mixed 2’OMe and 2′-F-RNA oligonucleotides includes a heating step (55 for at least 15 minutes) just prior to loading of the oligonucleotide solution onto the cartridge and greatly improves yield. Customers purifying longer oligos and those with inherent secondary structure have also used this slight change to the procedure successfully. Another example is the use of the DNA Glen-Pak for RNA desalting as an alternative to precipitation of 2′ deprotected, DMT-Off RNA oligonucleotides. As men.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
